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[actives] Jar not fully colonized and getting growth...im a total newb...not sure it I should birth this now or wait. Any advice? Ty!

[actives] Jar not fully colonized and getting growth...im a total newb...not sure it I should birth this now or wait. Any advice? Ty!

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Keefy_Nickles

Ok cool! Excellent info. I read a lot of different info regarding light. The growth has been slow but steady...its never stopped. Wet I would believe. It's my first attempt. Hopefully not bacteria! Only 6 of 10 jars showed any growth...I know I used too much of my syringes. And my substrate might have been too damp. 2 jars fully colonized out of ten...the 2 I did last colonized the best. This jar (pictured) I did in the second to last group.


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secret_identity88

>dialing your setup is half the fun 😉 Also half the frustration lol


danofworms

gotta earn those mushrooms ;)


Keefy_Nickles

Thank you!


AdditionalMind8989

I had this same thing happen to mine and because I was more than 98 % done I just brushed off the sub and went to fruiting. Just fyi also if you pick those mushies they're gonna be really strong! Mine were, I had some just like this and decided to eat them thinking I'm just gonna get a little body and visuals, then ended up going into outer space.


_Papagiorgio_

A tip I’ll offer is to stick the syringe tip at an angle through the media and against the glass and release until you see visible liquid come out and then stop. Do that it four locations mid way down. This is helpful because you can see when the pressure you’re applying on the plunger releases spores. Also flame sterilize between each jar.


Keefy_Nickles

Thank you! I tried to do this and didn't do well. I got it right on the last 2 jars lol. Oh my! I appreciate your help and will work on this.


Demoire

This right here - I test by keeping sterile jars and bags out in the open in random places for a month snd see if they remain sterile. I then know I can use that spot to incubate and the only real reason they should contam would be when I mess with them and open em etc I do this multiple times with multiple bags but yea, no difference between light and dark colonization - in fact, if anything a 12/12 of ambient light seems to increase colonization times.


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Demoire

Oh I got a flow hood so I absolutely hear ya on wanting one - I made the jump just a month or so ago. I absolutely love it. I have incubators which have expensive thermostats which dim the power to the heat instead of on and off so it keeps the temp precise, I use 12” flexwatt heat tape to surround the incubator. I have two of these but I’ve found it’s 100% unnecessary due to the reasons I mentioned above, in the summer. In the winter I’ll still need the heat, but I have fans n whatnot blasting all day on my jars (sterile jars, sterile LC prior to use and jars colonizing as well) and bags in the living room. Problem is it gets too hot elsewhere so I have to move everything to the living room. Working on installing a mini split ac system though, fucking expensive, just need to install it now lol


cannasav91

Is having a grow fan in a tent the same as a flow hood? I have a fan running and I have to turn it off to keep humidity level good, and that WITH a humidifier 🥴. The tops kept wanting to dry out but I finally found a way to keep it humid by shutting off my filtering fan :/ I just hope nothing gets contaminated


Demoire

Okay so your confusing a couple of things. A flow hood (laminar flow hood) is used in place of a still air box or a glove box and it provides sterile air to work in front of. This is the only time a vendor can claim sterility of a culture so long as it was prepared in front of a flow hood. Your fan is simply just a fan, a tool for bringing in fresh air and circulating the air in the tent to increase evaporation and improve pinning & pin sets. There absolutely is a balance between FAE and humidity so if your experiencing too much drying out, absolutely simply try reducing the amount of fresh air being brought in. Alternatively increase the humidity if needed.


cannasav91

Much appreciated for the response!


Demoire

Your welcome


jollierumsha

This is the answer. Also, there is some evidence that when grown in low light or no light there is increased psilocybin content Source:. https://psychedelicreview.com/cultivating-p-cubensis-light-and-tryptophan-are-key-for-controlling-psilocybin-levels/


gratefulyme

Source? Need something with non anecdotal sources and actual research beyond bioassay. Unfortunately this kind of rumor gets spread rapidly in the omc, so if we don't get evidence in 24 hours I'll remove this comment.


jollierumsha

https://psychedelicreview.com/cultivating-p-cubensis-light-and-tryptophan-are-key-for-controlling-psilocybin-levels/ Sorry, I didn't realize you guys were getting so academic up in here.


gratefulyme

Yes, when making scientific claims that will be repeated we do require scientific backing to prevent the spread of misinformation.


MycoloG-42

This


Ayelingboost

It's ready to be birthed, soaked, and Then FC!! Good luck


Keefy_Nickles

Awesome thank you. Should I just rinse off the uncolonized substrate before soaking? Ty!


Ayelingboost

I always pick the babies then soak. The uncolonized stuff will probably fall off.


Keefy_Nickles

Pick the babies? Will they not grow?


Ayelingboost

If you're going to soak them you have to pick them. At least that's what I've always been told.


Keefy_Nickles

Not doubting you...I wonder if someone can tell me why. Just curious.


WishboneThese3549

Wet mushrooms= rot and molding


Keefy_Nickles

Ok! Ty!


German9425

Yes, I’m sure it’ll come right off.


newthrowaway887

It’s okay. I’ve had plenty of cakes stall right on the bottom or right on the top it happens sometimes but regardless I’ve fruited those 90-95% colonized cakes with 100% success 🍄💪🏻 I accidentally put too much spore liquid into one and it only colonized about 60% but that sucker just dropped some chunky fruit


Joe-Burly

I’ve been reading about BRF neglect tek. RR states that you can get equivalent yields leaving them in the jars. Pretty interesting.


Medical-Woodpecker56

So how do you dunk the cakes if not taken out?


Joe-Burly

He dunks the cakes after the first flush and then puts them back in the jars. I saw some people say they just harvest and then put the cakes in the jars and fill with water for 24 hours.


Medical-Woodpecker56

I have some BFF cakes going rn, so thanks


Joe-Burly

Here is some info: https://mycotopia.net/archives/discus/messages/5/123431.html?1077396835


Joe-Burly

And a discussion/argument: https://www.shroomery.org/forums/showflat.php/Number/14395896


Keefy_Nickles

Hmmm...interesting


HugNikolas

Link plz


Joe-Burly

https://mycotopia.net/archives/discus/messages/5/123431.html?1077396835 Apparently some people got real mad about this stuff a while back.


HugNikolas

Lol silly humans taking alternate grow methods as personal attacks on their cultivation methods. Thanks for sharing.


Eyesinmyeyes00

Fruit it bro.


alphaminds

The question that comes to my mind is should they continue fruiting in vitro or put the s2b assuming the bottom of the grains has bacterial contam?


ElonMust888

are your inoculation holes covered with any type of filter? if there are, turn the jar upside down, if not, it's best to leave it as it is and just wait. Bottom portion lacks oxygen, because as mycelium grow and expel water, they block gap between glass and substrate. Once it's turned upside down, bottom portion can breath better and continue to colonize at normal pace.


Keefy_Nickles

Ok, I like the oxygen theory a lot better than the bacteria theory, not gonna lie, lol. I patted down the substrate when I filled the jars and later read that I probs shouldn't have. Could that contribute to the issue as well? Holes not covered. Thank you for your post!


ElonMust888

For some reason, many people like to give out "it's comtam!" advice without too much thinking or investigation and freak people out for no reason......... You are already using the "slender" jar that tend to pack too much on their own as they grow due to gravity, and packing them down while filling certainly doesn't help, so I am very certain they are just choking for air down there. Here's [how my jars look like](https://imgur.com/gallery/HPXz7FM), still got whole bunch of small air pockets left, where I pretty much just drop in substrate and get them into places with no packing at all. Even so loosely packed like this in a wide half pint jar, I see bottom struggling to breath towards to the end of colonization. if it's bacteria, bottom will turn to a slimy mushy soup by now due to decomposition, and they STINK like hot garbage, so you would definitely smell it by now. Also, the chance of bacteria being at bottom but not the top doesn't really make sense, based on how well the top developed, and no sign of myc piss fighting off bacteria infection on top. I've not had bacteria infection on my BRF yet, but from what I've seen, all the legit bacteria infection are all over the jar in patches. Do you have a still air box? if you do, put the jars in there, tear open all foil, wipe down the top with diluted bleach(preferred) or alcohol, and use few layers of microspore tapes or 20mm filter patches to cover up holes, and than turn them upside down, and give it a light tap so the substrate block drops onto the lid, and don't turn it again until it's ready for birth. Since your jars were covered by foil, chances are, not much containment have gotten in through the holes; and if they did, most are killed by the strong mycelium on top already. Use your own best judgement to decided if you want to turn them or not. For your next grow, set up jar lids like [THIS](https://imgur.com/gallery/hWb896a) on top, with 3 or 4 injection holes around the edge, one hole in center with [filter patch](https://www.amazon.com/Synthetic-Stickers-Mushroom-Applied-Cultivation/dp/B08P1T1Z1S/ref=sr_1_1_sspa?dchild=1&keywords=filter+patch&qid=1630096561&sr=8-1-spons&psc=1&spLa=ZW5jcnlwdGVkUXVhbGlmaWVyPUExU1YwUkhDSTk5T0JJJmVuY3J5cHRlZElkPUEwMTI4OTU2MlczNzBNVlExSUZUVSZlbmNyeXB0ZWRBZElkPUEwNDk4MTM4MlFCQkFTV05JTzExRSZ3aWRnZXROYW1lPXNwX2F0ZiZhY3Rpb249Y2xpY2tSZWRpcmVjdCZkb05vdExvZ0NsaWNrPXRydWU=) for gas exchange, and covered up all punctured holes with non breathable tape while they are inside SAB after inoculation, so you can readily turn them when things starts to slow down on bottom.


Keefy_Nickles

Hell yeah man! You are amazing. Thank you for taking the time to write that up. I'm planning to make a SAB this weekend. I need it for other stuff too. Thanks for the pics too!!!


ElonMust888

You are welcome buddy! Good luck!


ElonMust888

also, after you turned them upside down, new growth will be more fuzzy, so don't be alarmed by it. Here's a photo of [where my jar was stalling on the bottom, and how new growth looks like after it's turned.](https://imgur.com/gallery/VmLkYCj)


Keefy_Nickles

Super helpful! Thank you!


TheBE4S7

Also… your jars are mother effing huge lol stick to 1/2 pints if u can. Part of the issue (also the others mentioned above) is with a jar that size it’s going to take longer to colonize so by the time it gets near the bottom it’s already too wet and possibly contaminated-ish down there. If you’re going to stick to using that size of jar try laying them on their side and or rolling them periodically or flipping them head over heels from time to time


Keefy_Nickles

Thank you. However, these are 8oz jars (half pints). Pic must make it look huge.


SLTW562

FRUIT IT !


CosmicJenkum

My very first cakes were packed too tightly, slowing colonization. On the next batch, I left a little more air. This may or may not have anything to do with it, cant really see your substrate.


Keefy_Nickles

Ty!


therustynut

They just want to come out and say high.


Secret-Judgment3087

This same thing happened to a couple of my jars and I figured the bottoms were too wet but I birthed them and on day 2 now!


garbagethrowawayacou

This happened to me and it was because my Verm was too wet down there. Since it is fruiting, I would birth them, pick the fruits, then run the uncolonized part under water and gently wipe it off with your finger. It may be uncolonized in the middle of the jar also but that’s ok. After that you can put them in your chamber and fruit them like normal


Keefy_Nickles

Ty!


LegitimateMessage746

Typically, when a substrate that hasnt finished colonising starts to pin early, its because there is contam in the jar and the mycelium is rushing through the process. The best thing to do is, fruit it, wait and harvest as much of the good stuff as possible and throw out the rest before the jar gets too manky.


Kaufkins

If this is the case, isolate to avoid spreading any contamination.


Dude2daD

Wait until it’s fully colonized


Keefy_Nickles

Too late! I birthed it.


Cold-Injury

Light AND temp Will be blocked by aluminum foil. Making the top Warmer than the bottom because of moist inside the jar. Amaizing discovery


Keefy_Nickles

Oh man! Interesting!!! Something to definitely consider.


YogurtclosetNo5718

You can try to turn the jar upside down for a day or two. That has saved me a few times when this has happened.


Keefy_Nickles

Cool! Ty!


German9425

I’ll start by saying I’m no expert. But my guess is yes you should birth them. I believe your myc isn’t colonizing the bottom of your jars because there isn’t enough oxygen down there. Or possibly it’s started pinning because the jars are receiving too much light. I think if you leave those pins in there too long they’ll start to rot which will open your colony to contams.


MycoloG-42

Light doesn’t make pins. & they shouldn’t have growth unless they exposed the myc to fresh air. A lot of new people can’t keep the lids on and like to “check on it” when really you’re just going to start prematurely fruiting because you gave it the proper conditions. Edit: listen to what Althea said


Keefy_Nickles

Nope, never opened the lids.


MycoloG-42

That’s strange to me then. I’ve never experienced premie pins other than when I first started and would open em. I’d definitely say just take the foil off and let it do it’s thing. Maybe even take the cake out to check that bottom. If it’s too wet I’d just slice it off and put the good end in something else to fruit


Keefy_Nickles

Ok, awesome, ty! I appreciate your input!!


MycoloG-42

No problem!


xball89

I purchased some pre-sterilized substrate jars. They have fours holes in the top lid, which i used to inoculate through, and then a layer of aluminum foil wrapped around the lid. Should i remove this foil after inoculation and once jars are placed in the bin to colonize. Thanks!


MycoloG-42

Like, open holes? Or are there injection ports in the holes?


xball89

Just injector port holes. They are only a few millimeter in diameter at the most.


MycoloG-42

Ahh okay. I mean, Idk why the foil would be necessary so I’d probably just take it off


xball89

I was thinking to prevent against contamination following inoculation. I know it was placed there during sterilization of the jars, I assume to avoid soaking the BRF. I took it off, needle into each holt, then put it back on.


ExtortionWatchers

I've had pinning in jars before without opening. Sometimes due to a bacterial contam, and it will pop up sooner or later in my experience. I've had times also when there wasn't enough oxygen in them and I untwisted and retwisted the jar and everything came out fine also.


MycoloG-42

I’m no pro by any means by I’ve done plenty of tubs, So maybe I just haven’t run across it yet.


Keefy_Nickles

Ok, yeah, my jars may have gotten too much light. I don't want rot! I will birth them when I get home, thank you!


German9425

Best to keep them in dark while colonizing


Keefy_Nickles

Cool cool...there was a lot of conflicting info lol. But yeah, makes sense...


ptrkm

Let the shrooms grow, but without the aluminum foil. And then 2nd flush this cake. Btw. Have you shaken you jars?


jeremydkey1120

Thats only for whole grain spawn. This is a rice flour cake. You could shake it your whole life and the innards wouldn't move an inch.


ptrkm

Haha okay Didnt know that. Thought 'everything' had to be shaken. Thanks


basements_in_london

Why is the aluminum foil on top still during inoculations? You only needed it for the sterilization.


MoneyMike6666

What Tek are you using


rokketm3n

After you do a jar do some G2G it will spread it out since the others didn't catch


DueAardvark9488

Jars.you need shake it everyday until it get full colonized


th3_j0n_d03

Not tryna be a d*ck here but that’s terrible advice. Never shake BRF cakes and grain jars should only be shaken once. If u shake too much you will get stalled out jars Edits


Keefy_Nickles

Thank you for clarification!


Keefy_Nickles

I didn't shake it cause its a brf cake...I haven't done grain in jars yet. But I will this weekend! Excited.


bobjohnsonsburritos

no


luetennantdangledick

U should have stuck it in the fruiting chamber a long time ago lol